Replikacioni protein A1

RPA1

Available structures

PDB

Pretraga ortologa: PDBe RCSB

List of PDB id codes
1EWI, 1FGU, 1JMC, 1L1O, 2B29, 2B3G, 4IJH, 4IJL, 4IPC, 4IPD, 4IPG, 4IPH, 4LUO, 4LUV, 4LUZ, 4LW1, 4LWC, 4NB3, 4O0A, 4R4C, 4R4I, 4R4O, 4R4Q, 4R4T, 5E7N, 5EAY

Identifikatori

Alijasi

RPA1

Spoljašnji ID

OMIM: 179835 MGI: 1915525 HomoloGene: 2208 GeneCards: RPA1

Genska lokacija (miš)

Hr.	Chromosome 11 (mouse)^[1]

Band	11 B5\|11 45.79 cM	Start	75,188,992 bp^[1]
Band	11 B5\|11 45.79 cM	Kraj	75,239,150 bp^[1]

Genska ontologija
Molecular function	• DNA binding • metal ion binding • single-stranded DNA binding • damaged DNA binding • GO:0001948, GO:0016582 везивање за протеине плазме • nucleic acid binding • G-rich strand telomeric DNA binding • single-stranded telomeric DNA binding • sequence-specific DNA binding
Cellular component	• PML body • Нуклеоплазма • Replikacioni protein A • једро • site of DNA damage
Biological process	• DNA-dependent DNA replication • nucleotide-excision repair, DNA gap filling • DNA recombination • interstrand cross-link repair • error-free translesion synthesis • G1 phase • error-prone translesion synthesis • cellular response to DNA damage stimulus • репликација ДНК • regulation of cellular response to heat • DNA mismatch repair • translesion synthesis • transcription-coupled nucleotide-excision repair • nucleotide-excision repair, DNA incision • nucleotide-excision repair, DNA incision, 3'-to lesion • nucleotide-excision repair, DNA incision, 5'-to lesion • nucleotide-excision repair, preincision complex stabilization • nucleotide-excision repair • GO:0100026 поправка ДНК • base-excision repair • regulation of signal transduction by p53 class mediator • double-strand break repair via homologous recombination • nucleotide-excision repair, preincision complex assembly • protein localization to chromosome • telomere maintenance • telomere maintenance via semi-conservative replication • G1/S transition of mitotic cell cycle • DNA unwinding involved in DNA replication • telomere maintenance via telomerase • GO:0007126 мејоза
Sources:Amigo / QuickGO

Ortolozi

Vrste

Čovek

Miš

Entrez


6117


68275

Ensembl


ENSG00000132383


ENSMUSG00000000751

UniProt


P27694


Q8VEE4

RefSeq (mRNA)


NM_002945 NM_001355120 NM_001355121


NM_001164223 NM_026653

RefSeq (protein)


NP_002936 NP_001342049 NP_001342050


NP_001157695 NP_080929

Location (UCSC)

n/a

Chr 11: 75.19 – 75.24 Mb

PubMed search

^[2]

^[3]

Wikidata

View/Edit Human

View/Edit Mouse

Replikacioni protein A, 70 kDa DNK vezujuća podjedinica je protein koji je kod ljudi kodiran RPA1 genom.^[4]

Interakcije

Replikacioni protein A1 formira interakcije sa:

BRCA2,^[5]
BLM,^[6]^[7]^[8]
MCM2,^[9]
MCM4,^[9]
MCM6,^[9]
MCM7,^[9]
MUTYH,^[10]
ORC2L,^[9]
ORC6L,^[9]
P53,^[11]^[12]
RPA2,^[13]^[14]
RPA3,^[14]^[15]
TIPIN,^[16]
TP53BP1,^[17] i
XPA.^[18]

Reference

^ ^а ^б ^в GRCm38: Ensembl release 89: ENSMUSG00000000751 - Ensembl, May 2017
^ „Human PubMed Reference:”. National Center for Biotechnology Information, U.S. National Library of Medicine.
^ „Mouse PubMed Reference:”. National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Umbricht CB, Erdile LF, Jabs EW, Kelly TJ (1993). „Cloning, overexpression, and genomic mapping of the 14-kDa subunit of human replication protein A”. J Biol Chem. 268 (9): 6131—8. PMID 8454588.
^ Wong JM, Ionescu D, Ingles CJ (2003). „Interaction between BRCA2 and replication protein A is compromised by a cancer-predisposing mutation in BRCA2”. Oncogene. 22 (1): 28—33. PMID 12527904. doi:10.1038/sj.onc.1206071.
^ Brosh RM, Li JL, Kenny MK, Karow JK, Cooper MP, Kureekattil RP, Hickson ID, Bohr VA (2000). „Replication protein A physically interacts with the Bloom's syndrome protein and stimulates its helicase activity”. J. Biol. Chem. 275 (31): 23500—8. PMID 10825162. doi:10.1074/jbc.M001557200.
^ Opresko PL, von Kobbe C, Laine JP, Harrigan J, Hickson ID, Bohr VA (2002). „Telomere-binding protein TRF2 binds to and stimulates the Werner and Bloom syndrome helicases”. J. Biol. Chem. 277 (43): 41110—9. PMID 12181313. doi:10.1074/jbc.M205396200.
^ Moens PB, Kolas NK, Tarsounas M, Marcon E, Cohen PE, Spyropoulos B (2002). „The time course and chromosomal localization of recombination-related proteins at meiosis in the mouse are compatible with models that can resolve the early DNA-DNA interactions without reciprocal recombination”. J. Cell. Sci. 115 (Pt 8): 1611—22. PMID 11950880.
^ ^а ^б ^в ^г ^д ^ђ Kneissl M, Pütter V, Szalay AA, Grummt F (2003). „Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells”. J. Mol. Biol. 327 (1): 111—28. PMID 12614612. doi:10.1016/s0022-2836(03)00079-2.
^ Parker A, Gu Y, Mahoney W, Lee SH, Singh KK, Lu AL (2001). „Human homolog of the MutY repair protein (hMYH) physically interacts with proteins involved in long patch DNA base excision repair”. J. Biol. Chem. 276 (8): 5547—55. PMID 11092888. doi:10.1074/jbc.M008463200.
^ Romanova LY, Willers H, Blagosklonny MV, Powell SN (2004). „The interaction of p53 with replication protein A mediates suppression of homologous recombination”. Oncogene. 23 (56): 9025—33. PMID 15489903. doi:10.1038/sj.onc.1207982.
^ Riva F, Zuco V, Vink AA, Supino R, Prosperi E (2001). „UV-induced DNA incision and proliferating cell nuclear antigen recruitment to repair sites occur independently of p53-replication protein A interaction in p53 wild type and mutant ovarian carcinoma cells”. Carcinogenesis. 22 (12): 1971—8. PMID 11751427. doi:10.1093/carcin/22.12.1971.
^ Shao RG, Cao CX, Zhang H, Kohn KW, Wold MS, Pommier Y (1999). „Replication-mediated DNA damage by camptothecin induces phosphorylation of RPA by DNA-dependent protein kinase and dissociates RPA:DNA-PK complexes”. EMBO J. 18 (5): 1397—406. PMC 1171229 . PMID 10064605. doi:10.1093/emboj/18.5.1397.
^ ^а ^б Bochkareva E, Korolev S, Lees-Miller SP, Bochkarev A (2002). „Structure of the RPA trimerization core and its role in the multistep DNA-binding mechanism of RPA”. EMBO J. 21 (7): 1855—63. PMC 125950 . PMID 11927569. doi:10.1093/emboj/21.7.1855.
^ Bochkareva E, Frappier L, Edwards AM, Bochkarev A (1998). „The RPA32 subunit of human replication protein A contains a single-stranded DNA-binding domain”. J. Biol. Chem. 273 (7): 3932—6. PMID 9461578. doi:10.1074/jbc.273.7.3932.
^ Unsal-Kaçmaz K, Chastain PD, Qu PP, Minoo P, Cordeiro-Stone M, Sancar A, Kaufmann WK (2007). „The human Tim/Tipin complex coordinates an Intra-S checkpoint response to UV that slows replication fork displacement”. Mol. Cell. Biol. 27 (8): 3131—42. PMC 1899931 . PMID 17296725. doi:10.1128/MCB.02190-06.
^ Yoo E, Kim BU, Lee SY, Cho CH, Chung JH, Lee CH (2005). „53BP1 is associated with replication protein A and is required for RPA2 hyperphosphorylation following DNA damage”. Oncogene. 24 (35): 5423—30. PMID 15856006. doi:10.1038/sj.onc.1208710.
^ Li L, Lu X, Peterson CA, Legerski RJ (1995). „An interaction between the DNA repair factor XPA and replication protein A appears essential for nucleotide excision repair”. Mol. Cell. Biol. 15 (10): 5396—402. PMC 230789 . PMID 7565690. doi:10.1128/mcb.15.10.5396.

Literatura

Iftode C, Daniely Y, Borowiec JA (1999). „Replication protein A (RPA): the eukaryotic SSB.”. Crit. Rev. Biochem. Mol. Biol. 34 (3): 141—80. PMID 10473346. doi:10.1080/10409239991209255.
Binz SK, Sheehan AM, Wold MS (2005). „Replication protein A phosphorylation and the cellular response to DNA damage.”. DNA Repair (Amst.). 3 (8-9): 1015—24. PMID 15279788. doi:10.1016/j.dnarep.2004.03.028.
Dornreiter I, Erdile LF, Gilbert IU, von Winkler D, Kelly TJ, Fanning E (1992). „Interaction of DNA polymerase alpha-primase with cellular replication protein A and SV40 T antigen.”. EMBO J. 11 (2): 769—76. PMC 556510 . PMID 1311258.
Erdile LF, Heyer WD, Kolodner R, Kelly TJ (1991). „Characterization of a cDNA encoding the 70-kDa single-stranded DNA-binding subunit of human replication protein A and the role of the protein in DNA replication.”. J. Biol. Chem. 266 (18): 12090—8. PMID 2050703.
Li L, Lu X, Peterson CA, Legerski RJ (1995). „An interaction between the DNA repair factor XPA and replication protein A appears essential for nucleotide excision repair.”. Mol. Cell. Biol. 15 (10): 5396—402. PMC 230789 . PMID 7565690. doi:10.1128/mcb.15.10.5396.
Keshav KF, Chen C, Dutta A (1995). „Rpa4, a homolog of the 34-kilodalton subunit of the replication protein A complex.”. Mol. Cell. Biol. 15 (6): 3119—28. PMC 230543 . PMID 7760808. doi:10.1128/mcb.15.6.3119.
Umbricht CB, Griffin CA, Hawkins AL, Grzeschik KH, O'Connell P, Leach R, Green ED, Kelly TJ (1994). „High-resolution genomic mapping of the three human replication protein A genes (RPA1, RPA2, and RPA3).”. Genomics. 20 (2): 249—57. PMID 8020972. doi:10.1006/geno.1994.1161.
Gomes XV, Wold MS (1996). „Functional domains of the 70-kilodalton subunit of human replication protein A.”. Biochemistry. 35 (32): 10558—68. PMID 8756712. doi:10.1021/bi9607517.
Bochkarev A, Pfuetzner RA, Edwards AM, Frappier L (1997). „Structure of the single-stranded-DNA-binding domain of replication protein A bound to DNA.”. Nature. 385 (6612): 176—81. PMID 8990123. doi:10.1038/385176a0.
Amacker M, Hottiger M, Mossi R, Hübscher U (1997). „HIV-1 nucleocapsid protein and replication protein A influence the strand displacement DNA synthesis of lentiviral reverse transcriptase.”. AIDS. 11 (4): 534—6. PMID 9084803.
Cujec TP, Cho H, Maldonado E, Meyer J, Reinberg D, Peterlin BM (1997). „The human immunodeficiency virus transactivator Tat interacts with the RNA polymerase II holoenzyme.”. Mol. Cell. Biol. 17 (4): 1817—23. PMC 232028 . PMID 9121429.
Loor G, Zhang SJ, Zhang P, Toomey NL, Lee MY (1998). „Identification of DNA replication and cell cycle proteins that interact with PCNA.”. Nucleic Acids Res. 25 (24): 5041—6. PMC 147130 . PMID 9396813. doi:10.1093/nar/25.24.5041.
Bochkareva E, Frappier L, Edwards AM, Bochkarev A (1998). „The RPA32 subunit of human replication protein A contains a single-stranded DNA-binding domain.”. J. Biol. Chem. 273 (7): 3932—6. PMID 9461578. doi:10.1074/jbc.273.7.3932.
Golub EI, Gupta RC, Haaf T, Wold MS, Radding CM (1999). „Interaction of human rad51 recombination protein with single-stranded DNA binding protein, RPA.”. Nucleic Acids Res. 26 (23): 5388—93. PMC 148005 . PMID 9826763. doi:10.1093/nar/26.23.5388.
Shao RG, Cao CX, Zhang H, Kohn KW, Wold MS, Pommier Y (1999). „Replication-mediated DNA damage by camptothecin induces phosphorylation of RPA by DNA-dependent protein kinase and dissociates RPA:DNA-PK complexes.”. EMBO J. 18 (5): 1397—406. PMC 1171229 . PMID 10064605. doi:10.1093/emboj/18.5.1397.
Brosh RM, Orren DK, Nehlin JO, Ravn PH, Kenny MK, Machwe A, Bohr VA (1999). „Functional and physical interaction between WRN helicase and human replication protein A.”. J. Biol. Chem. 274 (26): 18341—50. PMID 10373438. doi:10.1074/jbc.274.26.18341.
Kim ST, Lim DS, Canman CE, Kastan MB (2000). „Substrate specificities and identification of putative substrates of ATM kinase family members.”. J. Biol. Chem. 274 (53): 37538—43. PMID 10608806. doi:10.1074/jbc.274.53.37538.
Brosh RM, Li JL, Kenny MK, Karow JK, Cooper MP, Kureekattil RP, Hickson ID, Bohr VA (2000). „Replication protein A physically interacts with the Bloom's syndrome protein and stimulates its helicase activity.”. J. Biol. Chem. 275 (31): 23500—8. PMID 10825162. doi:10.1074/jbc.M001557200.

PDB Galerija

1ewi: Ljudski replikacioni protein A: globalno savijanje N-terminala RPA-70 domena
1fgu: SSDNA vezujući domen velike podjedinice replikacionog proteina A
1jmc: Jednolančani DNK vezujući domen ljudskog replikacionog proteina vezanog za jednolančanu DNK
1l1o: Struktura trimerizacionog jezgra ljudskog replikacionog proteina A (RPA)
2b29: N-terminalni domen RPA70 podjednice ljudskog replikacionog proteina A.
2b3g: p53N (fragment 33-60) vezan za RPA70N

DNK replikacija (Prokariotska, Eukariotska)

Separacija
i inicijacija

Prokariotska (inicijacija)	Prereplikacioni kompleks Cdc6 Helikaza (dnaA, dnaB, T7) · Primaza (dnaG)
Eukariotska (priprema u G1 fazi)	Prereplikacioni kompleks: Kompleks prepoznavanja mesta početka (ORC1, ORC2, ORC3, ORC4, ORC5, ORC6) · Održavanje minihromozoma (MCM2 · MCM3 · MCM4 · MCM5 · MCM6 · MCM7) · CDC6 Faktor licenciranja Autonomna replikaciona sekvenca Protein jednolančanog vezivanja (SSBP1, SSBP2, SSBP3, SSBP4) RNaza H (RNASEH1, RNASEH2A) Helikaza: HFM1 Primaza: PRIM1 · PRIM2
Oba	Početak replikacije/Ori/Replikon Replikaciona viljuška (zaostajući i vodeći lanci) · Okazakijev fragment · Prajmer

Replikacija

Prokariotska (elongacija)	DNK polimeraza III holoenzim (dnaC, dnaE, dnaH, dnaN, dnaQ, dnaT, dnaX, holA, holB, holC, holD, holE) · Replizom · DNK ligaza · DNK stega · Topoizomeraza (DNK giraza) Prokariotska DNK polimeraza: DNK polimeraza I (Klenov fragment)
Eukariotska (sinteza u S fazi)	Replikacioni faktor C (RFC1) · Endonukleaza prepusta (FEN1) · Topoizomeraza · Replikacioni protein A (RPA1) Eukariotska DNK polimeraza: delta (POLD1, POLD2, POLD3, POLD4) DNK stega (PCNA)
Oba	Kretanje: Procesivnost · DNK ligaza

Terminacija

Telomer: Telomeraza (TERT, TERC, DKC1)

B bsyn: dnk (repl, cycl, reco, repr) · tscr (fact, tcrg, nucl, rnat, rept, ptts) · tltn (risu, pttl, nexn) · dnab, rnab/runp · stru (domn, 1°, 2°, 3°, 4°)